Blocking Solutions

aurion The signal-to-noise ratio determines the quality of any detection experiment. This ratio is in principle determined by the characteristics of specimen and detection reagents. With adequate blocking and incubation media, background reactions are minimized whereas specific reactions are not hampered.

Aurion has developed Blocking Solutions which effectively block ‘sticky’ surfaces by multi-point hydrophobic and charge-based interactions. The Blocking Solutions are specifically designed to meet the characteristics of each type of secondary conjugate.

Introduction
The AURION Blocking Solutions are used to prevent immunoreagents from binding non-specifically to specimens with “sticky” surface properties.

Procedures to eliminate background comprise three main steps:

  1. To suppress residual aldehyde activity
  2. To saturate multipoint hydrophobic moieties and positive charges with high molecular weight compounds such as those present in the AURION Blocking Solutions
  3. To reduce aspecific binding of immunoreagents caused by hydrophilic interaction with competing molecules in the incubation and washing solution. AURION BSA-c™ is a particularly effective reagent for this purpose.
These steps should be balanced for optimum results.

Product Description
AURION Blocking Solutions are prepared using specially selected compounds. All ruminant proteins are obtained from healthy livestock.

AURION Blocking solutions contain Bovine Serum Albumin and Cold Water Fish Skin Gelatine in phosphate buffered saline with sodium azide as preservative. Normal serum may have been added as indicated on the label. The blocking capacity of each lot is determined using a dot-spot test system as described by Moeremans et al., J. Immunol. Methods 74, (1984), 353.
Each package contains 30 ml of solution. It accommodates 300 specimens for light microscopy at 100 µl/specimen (~ 3 drops), or 1000 EM grids at 30 µl/specimen (~ 1 drop).

Application Instructions
Detailed information is provided on the package inserts.

Electron Microscopy

On-Grid Labeling
Placing grids on top of drops of Blocking Solution arrayed on a sheet of Parafilm™ after they have been treated for aldehyde inactivation.

Pre-embedding Labeling
Floating specimens in Blocking Solution, preferably on a rocking table.

Light Microscopy

On-slide or -coverslip Labeling
Applying a few drops of Blocking Solution to cover the specimen.

Bio Assays
Incubating specimens (e.g. immunoblot strips) in Blocking Solution on a rocking table in screw-cap sealed disposable tubes.
Specificity

Blocking Solutions are available in the following specificities:


Storage
AURION Blocking Solutions have a guaranteed shelf life of 18 months from the date of quality control analysis.
The products should be stored at 4-8°C. Freezing is not recommended.

© Aurion 2007 - 2010

Features of Aurion Blocking Solutions

  • ready-to-use solutions
  • for immunohisto and -cytochemistry
  • for immunogold detection systems
  • for enzyme based detection systems
  • for fluorescent detection systems
  • easy application with dropper bottle
  • economical use, no left-overs
  • tailored versions
  • for EM grids and LM specimens
  • for bio assays
Price Information      
For Protein A/G immuno reagents:
905.001 - 30 ml - € 43.00

For Goat immuno reagents:
905.002 - 30 ml - € 74.00

For Rabbit immuno reagents:
905.003 - 30 ml - € 86.00

For Sheep immuno reagents:
905.004 - 30 ml - € 74.00

For Donkey immuno reagents:
905.005 - 30 ml - € 86.00


Prices Ex VAT (if applicable)

Material Safety Data Sheet
AURION blocking solutions: MSDS or technical data sheet


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