The signal-to-noise ratio determines the
quality of any detection experiment. This ratio is in principle determined by
the characteristics of specimen and detection reagents. With adequate blocking
and incubation media, background reactions are minimized whereas specific
reactions are not hampered.
Aurion has developed Blocking Solutions which
effectively block ‘sticky’ surfaces by multi-point hydrophobic and
charge-based interactions. The Blocking Solutions are specifically designed to
meet the characteristics of each type of secondary conjugate.
The AURION Blocking Solutions are used to prevent
immunoreagents from binding non-specifically to specimens with
“sticky” surface properties.
Procedures to eliminate
background comprise three main steps:
- To suppress residual aldehyde
- To saturate multipoint hydrophobic moieties and positive
charges with high molecular weight compounds such as those present in the AURION
- To reduce aspecific binding of immunoreagents caused
by hydrophilic interaction with competing molecules in the incubation and
washing solution. AURION BSA-c™ is a particularly effective reagent for
These steps should be balanced for optimum
AURION Blocking Solutions are prepared using
specially selected compounds. All ruminant proteins are obtained from healthy
AURION Blocking solutions contain Bovine Serum Albumin and
Cold Water Fish Skin Gelatine in phosphate buffered saline with sodium azide as
preservative. Normal serum may have been added as indicated on the label. The
blocking capacity of each lot is determined using a dot-spot test system as
described by Moeremans et al., J. Immunol. Methods 74, (1984), 353.
package contains 30 ml of solution. It accommodates 300 specimens for light
microscopy at 100 µl/specimen (~ 3 drops), or 1000 EM grids at 30
µl/specimen (~ 1 drop).Application
Detailed information is provided on the package
Placing grids on top of drops of
Blocking Solution arrayed on a sheet of Parafilm™ after they have been
treated for aldehyde inactivation.
Floating specimens in Blocking
Solution, preferably on a rocking table.Light
Applying a few drops of Blocking Solution to
cover the specimen.
Incubating specimens (e.g. immunoblot strips) in Blocking
Solution on a rocking table in screw-cap sealed disposable
Blocking Solutions are available in the following
- serum-free: for use with Protein A and Protein G
- with Normal Goat serum: for use with reagents based on
secondary antibodies raised in Goat.
- with Normal Rabbit serum: for use
with reagents based on secondary antibodies raised in Rabbit.
Normal Sheep serum: for use with reagents based on secondary antibodies raised
- with Normal Donkey serum: for use with reagents based on
secondary antibodies raised in Donkey.
AURION Blocking Solutions have a
guaranteed shelf life of 18 months from the date of quality control
The products should be stored at 4-8°C. Freezing is not
© Aurion 2007 - 2010