Colloidal gold based protein Stain
Col-Aurion is a colloidal gold particle based
total protein stain, developed for the sensitive staining of electrophoretically
separated protein bands on nitrocellulose or PVDF™ blotting membranes.
Colloidal gold particles accumulate at the site of the protein bands on the
membrane, generating an intense dark red staining pattern. The total protein
stain assists in assessing immunoblotting results and to evaluate the
effectivity of the blotting procedure.
Introduction
The negative surface charge of
colloidal gold particles is responsible for their high affinity for positively
charged macromolecules. This characteristic was the basis for the development
of a total protein stain based on colloidal gold.
Product Description
Col-Aurion
is a total protein stain consisting of a solution of coated gold particles with
an average particle diameter of 15 nm. The staining principle is based on the
electrostatic binding of negatively charged gold particles to proteins with a
positive charge present at low pH.
A unique feature of Col-Aurion is the use
of BSA-c™ to shield off the surface of the gold particles. Destabilization
of gold particles that might occur as a result of interaction with detached
protein is thus prevented. The strong negative charge of BSA-c™ gives an
additional increase in sensitivity of the stain.
Col-Aurion total protein
stain has a pH of 3.2 and is ready to use. For removal of surplus of weakly
bound protein from the membrane, a 10 ml vial of Tween-20™ is
included.
Application Instructions
Detailed information is provided in
the package insert.Col-Aurion is a sensitive total protein stain for bio
assays, especially nitrocellulose and PVDF Western blots and dot-blots. The
protein binding substratum has to carry a negative charge.
Storage
Col-Aurion has a guaranteed shelf life of 18 months
from the date of quality control analysis. Store at 4-8°C. Do not
freeze.
Features of Col-Aurion
- ready-to-use solution
- of BSA-c™ stabilized colloidal gold particles
- for total protein staining on membranes
- with high sensitivity, no overstaining
- intense and permanent signal
- straightforward method
- economical use
600.011 - Col-Aurion - 500 ml - € 109,00
