Custom Labeling Service
Introduction
The standard series of AURION immunogold reagents
is intended for the two and three step detection of antigens. Although these
approaches cover the majority of all applications there may occasionally exist
a need for a direct label where the gold particles are coupled directly to the
primary antibody or, more general, to a protein or peptide with specific
binding properties. An example is found in working with specimens where
substantial amounts of homologous immunoglobulins are present. A further
example is found in the detection of receptors through labeled ligand
molecules.
To fulfill these needs AURION offers a custom labeling
service for antibodies, antibody fragments, proteins, and peptides with gold
particles of any requested size, including ultra small gold particles. AURION
designs and manufactures such custom conjugates in close co-operation and in
agreement with the principal investigator.
Starting material and quality control
criteria
Our custom labeling service is based on the coupling of up
to 1 mg of protein or ligand*.
The custom prepared product is initially
quality controled by AURION according to our in-house standards for instance
with respect to size distribution and clustering. In agreement with the
principal investigator the binding substance’s specific quality control
criteria are determined. These are tailored for each individual assignment. As
a matter of course the custom conjugate is approved only when both sets of
quality control criteria have been met.
A few examples
AURION successfully
conjugated a number of polyclonal antibodies, mouse monoclonal antibodies,
F(ab’)2 and F(ab) fragments, high and low density lipoproteins, as well
as peptides such as an Angiotensin derivative (octapeptide), Interleukin II,
Tumor Necrosis Factor and a number of peptide hormones. So far the efficiency
of coupling for the above mentioned custom conjugates has been greater than 70%
on all occasions, while efficiencies up to 90% are no exception.
No cure, no
pay
The custom labeling service is on the basis of “no cure, no
pay”, with the exception that a small intake fee applies to each actual
laboratory attempt. We invite you to contact us if you request information
which is more tailored to your specific research applications.
* As a
rule AURION will only accept pure substances as starting material, either
lyophilized or dissolved in a medium of known chemical composition. Interfering
substances must not be present. Nucleic acids are not accepted, since they can
not be conjugated to colloidal gold particles while retaining
activity.
Features of AURION Custom Labeling Service
- For one step detection
- based on the coupling of 1 mg of
protein or ligand to a particle size of your choice
- In close co-operation custom designed and manufactured
- No cure, no pay
Price Information
900.055 - 1 mg antibody coupled to gold particle - € 949,00
