Custom Labeling Service
Introduction
The standard series of AURION immunogold reagents
is intended for the two and three step detection of antigens. Although these
approaches cover the majority of all applications there may occasionally exist a
need for a direct label where the gold particles are coupled directly to the
primary antibody or, more general, to a protein or peptide with specific binding
properties. An example is found in working with specimens where substantial
amounts of homologous immunoglobulins are present. A further example is found in
the detection of receptors through labeled ligand molecules.
To fulfill
these needs AURION offers a custom labeling service for antibodies, antibody
fragments, proteins, and peptides with gold particles of any requested size,
including ultra small gold particles. AURION designs and manufactures such
custom conjugates in close co-operation and in agreement with the principal
investigator.
Starting material and quality control criteria
Our custom
labeling service is based on the coupling of up to 1 mg of protein or
ligand*.
The custom prepared product is initially quality controled by AURION
according to our in-house standards for instance with respect to size
distribution and clustering. In agreement with the principal investigator the
binding substance’s specific quality control criteria are determined.
These are tailored for each individual assignment. As a matter of course the
custom conjugate is approved only when both sets of quality control criteria
have been met.
A few examples
AURION successfully conjugated a number of
polyclonal antibodies, mouse monoclonal antibodies, F(ab’)2 and F(ab)
fragments, high and low density lipoproteins, as well as peptides such as an
Angiotensin derivative (octapeptide), Interleukin II, Tumor Necrosis Factor and
a number of peptide hormones. So far the efficiency of coupling for the above
mentioned custom conjugates has been greater than 70% on all occasions, while
efficiencies up to 90% are no exception.
No cure, no pay
The custom labeling
service is on the basis of “no cure, no pay”, with the exception
that a small intake fee applies to each actual laboratory attempt. We invite you
to contact us if you request information which is more tailored to your specific
research applications.
* As a rule AURION will only accept pure
substances as starting material, either lyophilized or dissolved in a medium of
known chemical composition. Interfering substances must not be present. Nucleic
acids are not accepted, since they can not be conjugated to colloidal gold
particles while retaining activity.
Features of AURION Custom Labeling Service
- For one step detection
- based on the coupling of 1 mg of
protein or ligand to a particle size of your choice
- In close co-operation custom designed and manufactured
- No cure, no pay
Price Information
900.055 - 1 mg antibody coupled to gold particle - € 995,00
