To prepare a high quality (immuno)gold
conjugate it is important to have particles with uniform size and highly
adsorptive surfaces.
The Aurion gold sols are prepared according to unique
production protocols. This provides for sol particles with the same narrow size
distribution and adsorption properties as employed in the Conventional
Immunogold Reagents.
Introduction
The preparation of conventional gold
reagents is based on gold particles with a diameter suited for direct electron
microscopic visualization. Aurion offers a range of Conventional Immunogold
Reagents which cover the majority of approaches in transmission and scanning
electron microscopy.
Aurion Gold Sols (solutions of high quality
unconjugated gold particles) provide opportunities for users to prepare
conjugates with primary antibodies, ligands and other binding agents with the
same particle characteristics as in the Conventional Immunogold Reagents.
Product
Description
AURION Gold Sols are prepared according to a unique
protocol, warranting narrow size distribution and reproducible adsorption
characteristics.
The AURION gold sols are available in the same size range
as the Conventional Immunogold Reagents: 6, 10, 15 and 25 nm. The particle
population is monodisperse and thus shows minimal size variation and overlap.
Typically, the coefficient of variance for the 6 and 25 nm particle size sols
is less than 12%, whereas the 10 and 15 nm size sols show less then 10%
variation. Actual lot specifications (size, variation and expiry date) are
indicated on the accompanying package insert.
Package size: 100 ml of high
quality gold sol at an OD520nm of approximately 1.
Storage
AURION gold sols have
a guaranteed shelf life of 12 months from the date of quality control
analysis.
The products should be stored at 4-8°C.
Freezing is not
recommended.
Application Instructions
Detailed instructions for conjugate
preparation and evaluation are provided in the package inserts.