Gold Tracers
Aurion Gold Tracers are used to visualize
charged moieties in specimens: anionic tracers bind to polycationic moieties
(basic proteins e.g. histones, cationic tracers bind to polyanionic moieties
(membranes, acidic proteins). “Neutral” BSA coated gold tracers are
useful for detecting open connections and tissue damage. Under proper conditions
and with suitable microscopical techniques the tracers can also be used to
follow cellular events in time.
Introduction
The AURION Anionic and Cationic Gold Tracers
are designed to detect charged areas; the anionic tracers detecting multiple
positive charge moieties, the cationic tracers detecting multiple negative
charges.
Product description
The AURION Gold Tracers are available in
the full range of particle sizes: Ultra Small, 6, 10, 15 and 25 nm. Anionic
Gold Tracers are prepared with BSA-c™ as particle conjugated protein.
Cationic Gold Tracers are prepared with methylated BSA. AURION Gold Tracers are
available in 5 and 10 ml volume packages and are supplied at an OD520nm of 2.0
for the conventional particle size range and at equivalent OD for the Ultra
Small tracers.
The products are supplied in PBS, with 15 mM
NaN3.
NOTE
1:
AURION Gold Tracers are also available in bulk and at different
optical density if required.
NOTE 2:
AURION Gold Tracers are shipped
containing NaN3 as preservative. If they are intended for use in living
organisms, the preservative has to be removed prior to use. This can be
achieved either by dialysis or by buffer exchange using for instance a
Pharmacia PD-10 column.
Application Instructions
In order to obtain a
positive reaction when using these tracers the following should be kept in
mind:
- charge interactions are influenced by the presence of ions, di- and trivalent ions being of more influence than univalent ions. The influence is concentration dependent. Binding is stronger in low ionic-strength media.
- charge interactions are influenced by pH. The charge of components in the specimen is dependent of their isoëlectric point, below the IEP the charge is positive, above the IEP negative. Significant binding will only occur at a pH at least one to two pH-units higher or lower than the IEP.
Cationic tracers are positively charged at pH <10
BSA tracers are intended for tracking fine capillaries or connecting spaces in intact tissues.
Storage
Aurion gold tracers have a guaranteed shelf life of 18 months from the date of quality control analysis.
The products should be stored at 4-8°C.
Freezing is not recommended.
Features of Aurion Gold Tracers
- Cationic, anionic and BSA tracers
- available with conventional particle sizes
- available with ultra small particle sizes
- for the microscopical detection of: charged moieties, open connections and tissue damage, cellular dynamic events (event tracking)
Follow the link of the gold tracer of your choice below, to find information about sizes and prices:
Cationic Tracers
Anionic Tracers
BSA Tracers
Material Safety Data Sheet
download the MSDS in pdf-format by following the link below:
AURION Gold Tracers
