How to control background in a nut shell

Background Is Controlled by Three Independent Steps

1. Low Molecular Weight Block
(before protein block)

Purpose: to inactivate residual fixative e.g. aldehydes, using

amino acids such as glycin or lysin, or
aldehyde inactivating compounds such as NaBH4 and NH2OH

2. High Molecular Weight Protein Block
(before immunolabeling)

Purpose: to prevent stickiness to hydrophobic areas and domains with excessive positive charges based on multiple point interactions (high affinity protein binding capacity), using

albumin
normal serum

3. Incubation and Wash solution
(during immunolabelling)

Purpose: to eliminate aspecific binding of immunoconjugates based on hydrophilic interactions ("oligo"point interactions) by competition, using

acetylated albumin (AURION BSA-c™) in the incubation buffer

Abandoning BSA-c™ molecules compete with antibodies and secondary immuno reagents for aspecific binding to positively charged specimen compounds, thus minimizing background.

Please refer to our Newsletter 1 and Newsflyer 1 for more information on the subject of background.
You can find incubation protocols in "The Aurion method" section of this website.

The Aurion Method

clean and clear results
reproducible and reliable
synergistic products
optimal signal to noise ratio

AURION Products

_ HOME _ Sharing_our_knowledge _ How To Control Background