In many cases double labeling experiments are based on
using two primary antibodies from different animal species, for instance mouse
and rabbit. Of late we noticed that an increasing number of users have primary
antibodies developed in Goat. It was impossible to perform a double label
experiment in combination with for instance a mouse primary antibody, since
applicable secondary antibodies are either raised in Goat or in Rabbit. Hence
the Goat-anti-mouse would be recognized by the Rabbit-anti-Goat, and it would
be impossible to get reliable results. In the past, only using directly labeled
primary antibodies would solve this. And this would require researchers to go
through the lengthy procedure of preparing such conjugates, and then often with
very limited success.
To overcome this difficulty Aurion has developed a new series of secondary immunogold conjugates based on antibodies raised in Donkey, including Donkey-anti-Goat. It will be obvious that mixing a Donkey-anti-Goat and a Donkey-anti-Mouse will provide a successful approach in the aforementioned case.
The Donkey Immuno Gold conjugates are available in the sizes Ultra Small and Conventional.