Dotspottest

aurion AURION Newsletter 4
THE DOT-SPOT TEST: a simple method to monitor immunoreagent reactivity and influence of fixation on antigen recognition
P.F.E.M. van de Plas

This newsletter focuses on the ImmunoGold Silver Staining method. However, the dot-spot test can be used for every immunodetection system giving signals that are macroscopically visible. Examples are immuno-enzymatic methods using peroxidase or alkaline phosphatase as marker molecule and carbon or latex coated immunochemicals.

Topics

Analysis of critical steps in immunodetection studies
Setting up a dot-spot test
Pin-pointing problems through simple dot-spot experiments
How to test
silver enhancement reagents
immuno conjugates
antigen recognition
influence of fixation
Comprehensive and detailed description of methods
Schematical trouble-shooter

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