Reactivity of Protein A and Protein G
Membrane labeling with protein A 15 nm gold
conjugate.
Courtesy of T.Wakefield, University of Auburn, Alabama
Species Ig(sub)class Protein A
Protein G
Rabbit
Ig
++ ++
Mouse
IgG1
- +
IgG2a
+ +
IgG2b
+ +
IgG3
+ ++
IgA
- ?
IgM
- ?
Rat
IgG1
- ?
IgG2a
- ++
IgG2b
- +
IgG2c
+ +
IgM
- ?
Man
IgG1
++ ++
IgG2
++ ++
IgG3
- ++
IgG4
++ ++
IgA
- -
IgM
+ -
IgD
- -
Goat
Ig
- ++
Sheep
Ig
- ++
Guinea Pig
Ig
++
+
Chicken
Ig
- -
Pig
Ig
++
+
Horse
Ig
- ++
Cow
Ig
+ ++
Dog
Ig
+
+
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