AURION: Post embedding Silver Enhancemen

Silver Enhancement Procedure

aurion using AURION R-Gent SE-EM
(Silver Enhancement for Electron Microscopy)

It is assumed that the Developer has been prepared as prescribed.

1. Prepare the enhancement mixture as follows:
Once the DEVELOPER and ENHANCER have reached room temperature, give 20 drops of the ENHANCER solution into a vial that will contain at least 1.5 ml, e.g. an Eppendorf vial. Make sure to keep the bottle upside down in a vertical position. Add 1 drop of the DEVELOPER solution, again making sure that the bottle is kept upside down in a vertical position. Mix well on a vortex.

2. Grids are floated on drops of enhancement mixture. Enhancement time is typically between 30 minutes and 1 hour at room temperature (preferably 20°C). The actual enhancement time has to be established empirically and adjusted according to the desired particle growth.

3. When enhancement is complete the grids are washed extensively on drops of distilled water (at least 3x10 minutes). A postfixation with photographic fixer is not required.

4. Grids are contrasted according to standard procedures.

RECOMMENDED FOR EM

Silver enhancement for EM
(when using Ultra Small IGR)

AURION R-Gent SE-EM

For post-embedding
Glycine to prepare the aldeyde blocking solution
For pre-embedding
Sodium Borohydride to prepare the aldehyde blocking solution
Triton-X-100 for increased accessibility

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